Report研究成果
2024年
Cui, M., Yamano, K., Yamamoto, K., Yamamoto-Imoto, H., Minami, S., Yamamoto, T., Matsui, S., Kaminishi, T., Shima, T., Ogura, M., Tsuchiya, M., Nishino, K., Layden B., Kato, H., Ogawa, H., Oki, S., Okada, Y., Isaka, Y., Kosako, H., Matsuda, N., *Yoshimori, T., *Nakamura, S. HKDC1, a novel target of TFEB, is essential to maintain both mitochondrial and lysosomal homeostasis, preventing cellular senescence.
PNAS, 121, e2306454120 (2024).
プレスリリース
https://www.naramed-u.ac.jp/university/kenkyu-sangakukan/oshirase/r5nendo/tfeb.html
Kakuda, K., Ikenaka, K., Kuma, A., Doi, J., Aguirre, C., Wang, N., Ajiki, T., Choong, C., Kimura, Y., Mohamad, S., Badawy, M., Shima, T., Nakamura, S., Baba, K., Nagano, S., Nagai, Y., Yoshimori, T., Mochizuki, H. Lysophagy protects against propagation of α-synuclein aggregation through ruptured lysosomal vesicles.
PNAS, 121, e2312306120 (2024).
Zhang, W., Nishimura, T., Tooze SA. Real-Time Monitoring of ATG8 Lipidation in vitro Using Fluorescence Spectroscopy.
Bio Protoc., 14, e4917. (2024).
https://bio-protocol.org/en/bpdetail?id=4917&type=0
2023年
Ogura, M., Kaminishi, T., Shima, T., Torigata, M., Bekku, N., Tabata, K., Minami, S., Nishino, K., Nezu, A., Hamasaki, M., Kosako, H., *Yoshimori, T., *Nakamura, S. Microautophagy regulated by STK38 and GABARAPs is essential to repair lysosomes and prevent aging.
EMBO Rep, e57300, (2023).
プレスリリース
https://www.naramed-u.ac.jp/university/kenkyu-sangakukan/oshirase/r5nendo/microautophagy.html
Shioda, T., Ikenaka, K., Fujita, N., Kanki, T., Oka, T., Mochizuki, H., Antebi, A., *Yoshimori, T., *Nakamura, S. Neuronal MML-1/MXL-2 regulates systemic aging via glutamate transporter and cell non-autonomous autophagic and peroxidase activity. PNAS, 120, e2221553120, (2023).
プレスリリース
https://www.naramed-u.ac.jp/university/kenkyu-sangakukan/oshirase/r5nendo/autophagy.html
Nishimura, T., Lazzeri, G., Tooze, S.A., *Covino, R. ATG3 proteins possess a unique amphipathic α-helix essential for the Atg8/LC3 lipidation reaction. Autophagy, Sep 7, 1-2. (2023).
*Tooze, S.A., Zhang, W., Lazzeri, G., Gahlot, D., Thukral, L., Covino, R., Nishimura, T. Membrane association of the ATG8 conjugation machinery emerges as a key regulatory feature for autophagosome biogenesis. FEBS Lett. Jun 1. (2023). doi: 10.1002/1873-3468.14676.
*,#Nishimura, T., #Lazzeri, G., Mizushima, N., *Covino, R., Tooze, S.A.. Unique Amphipathic a-helix Drives Membrane Insertion and Enzymatic Activity of ATG3. Sci. Adv., 9(25), eadh1281. (2023).
東大プレスリリースのリンク
https://www.u-tokyo.ac.jp/content/400217609.pdf
#Zhang, W., #Nishimura, T., Gahlot, D., Saito, C., Davis, C., Jefferies, H.B.J., Schreiber, A., Thukral, L., *Tooze SA. Autophagosome membrane expansion is mediated by the N-terminus and cis-membrane association of human ATG8s. eLife, e89185. (2023).
中村修平「概論 -種々の生命現象に関わるリソソームの新たな姿-」実験医学 7月号 2023 (リソソーム特集企画を担当)
小倉もな美、吉森 保、中村修平「リソソーム損傷応答とその生理学的意義」実験医学 7月号 2023
中村 修平、 井本 ひとみ、 吉森 保「(抗老化医療の未来をさぐる:哺乳類における老化・寿命制御の理解とその社会実装) オートファジーと老化」Geriatric Medicine 61(1) 45-49 2023年
2022年
Yamamoto-Imoto, H., Minami, S., Shioda, T., Yamashita, Y., Sakai, S., Maeda, S., Yamamoto, T., Oki, S., Takashima, M., Yamamuro, T., Yanagawa, K., Edahiro, R., Iwatani, M., So, M., Tokumura, A., Abe, T., Imamura, R., Nonomura, N., Okada, Y., Ayer, E. D., Ogawa, H., Hara, E., Takabatake, Y., Isaka, Y., *Nakamura S., *Yoshimori T. Age-associated decline of MondoA drives cellular senescence through impaired autophagy and mitochondrial homeostasis. Cell Rep, 38, 110444, (2022)
Yamamuro, T., *Nakamura S., Yanagawa, K., Tokumura, A., Kawabata, T., Fukuhara, A., Teranishi, H., Hamasaki, M., Shimomura, I., *Yoshimori, T., Loss of Rubicon in adipocytes mediates the upregulation of autophagy to promote the fasting response. Autophagy, 14, 1-11, (2022).
Murakawa, T., Nakamura, T., Kawaguchi, K., Murayama, F., Zhao, N., Stasevich, T., Kimura, H., Fujita, N.
A Drosophila Toolkit for HA-tagged Proteins Unveiled a Block in Autophagy Flux in the Last Instar Larval Fat Body.
Development (2022) 149 (6): dev200243
https://doi.org/10.1242/dev.200243
蛹期の擬似的な飢餓に備えてショウジョウバエ幼虫脂肪体のリソソーム活性が抑制されることを示す成果がDevelopmentに掲載されました。
プレスリリース
https://www.titech.ac.jp/news/2022/063216
Ogura, M., Shima, T., Yoshimori, T., *Nakamura, S. Protocols to monitor TFEB activation following lysosomal damage in cultured cells using microscopy and immunoblotting, STAR Protoc. 3, 101018, (2022)
https://hivebench-star-protocols.s3.amazonaws.com/protocols/1290.pdf
オートファジー・リソソーム生合成のマスター転写因子TFEBの活性をモニターするプロトコル論文がSTAR Protocolsに掲載されました。
Oe, Y., Kakuda, K., Yoshimura, S., Hara, N., Hasegawa, J., Terawaki, S., Kimura, Y., Ikenaka, K., Suetsugu, S., Mochizuki, H., *Yoshimori, T., *Nakamura, S. PACSIN1 is indispensable for amphisome-lysosome fusion during basal autophagy and subsets of selective autophagy. PLoS Genet., 18(6), e1010264, (2022)
プレスリリース
https://resou.osaka-u.ac.jp/ja/research/2022/20220701_1
Tsujimoto, K., Jo, T., Nagira, D., Konaka, H., Park,,JH., Yoshimura, SI., Ninomiya, A., Sugihara, F., Hirayama, T., Itotagawa, E., Matsuzaki, Y., Takaichi, Y., Aoki, W., Saita, S., Nakamura, S., Ballabio, A., Nada, S., Okada, M., Takamatsu, H., *Kumanogoh, A. EMBO J., (2022), e111389.
Jia, X., Knyazeva, A., Zhang, Y., Castro-Gonzalez, S., Nakamura, S., Carlson, L.A., Yoshimori, T., Corkery, D.P., Wu, YW. V. cholerae MakA is a cholesterol-binding pore-forming toxin that induces non-canonical autophagy. J Cell Biol., 221, e202206040, (2022)
Oginuma, M., Nishida, M., Ohmura-Adachi, T., Abe, K., Ogamino, S., Mogi, C., Matsui, H., Ishitani, T. Rapid reverse genetics systems for Nothobranchius furzeri, a suitable model organism to study vertebrate aging.
Scientific Reports, 12: 11628 (2022)
プレスリリース
http://www.biken.osaka-u.ac.jp/achievement/research/2022/178
*Taki Nishimura, Gianmarco Lazzeri, Noboru Mizushima, Roberto Covino, *Sharon A. Tooze. Unique Amphipathic α-helix Drives Membrane Insertion and Enzymatic Activity of ATG3. bioRxiv 2023.02.11.528101; doi: https://doi.org/10.1101/2023.02.11.528101
*Nakamura, S., Shioda, T., *Yoshimori, T. Autophagy in aging and longevity (Chapter12), Aging Mechanism II: Longevity, Metabolism and Brain Aging (Springer) (2022)
Cui, M., *Yoshimori, T., *Nakamura, S. Autophagy system as a potential therapeutic target for neurodegenerative diseases. 155, 105308, Neurochem Int, (2022)
*Nakamura, S., Akayama, S., *Yoshimori, T. Non-canonical roles of ATG8 for TFEB activation. Biochem Soc Trans. 50, 47-54, (2022)
Minami, S., *Nakamura, S., *Yoshimori, T. Rubicon in Metabolic Disease and Aging. Front. Cell Dev. Biol., 9, 816829, (2022)
中村修平、吉森保「オートファジーと老化・神経変性疾患」老年精神医学雑誌 33, 79-86, 2022年
Oe, Y., *Yoshimori, T., *Nakamura, S. Novel insights into the molecular mechanism of amphisome-lysosome fusion. Autophagy Reports, 1, 542-545, (2022)
*Nakamura, S., Shioda, T., *Yoshimori, T. Autophagy in aging and longevity (Chapter11), Aging Mechanism II: Longevity, Metabolism and Brain Aging (Springer) (2022)
Yamamoto-Imoto, H., Hara, E., *Nakamura, S., *Yoshimori, T. Measurement of autophagy via LC3 western blotting following DNA-damage-induced senescence. STAR Protoc., 3, 101539, (2022)
小倉もな美、吉森 保、中村修平 「損傷リソソーム応答によるリソソーム恒常性の維持機構」生体の科学 73, 3, 221-225, 2022年
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